# What do you think would happen if you failed to vortex your homogenate sample before removing...

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What do you think would happen if you failed to vortex your homogenate sample before removing an aliquot for an assay? In this experiment, we sought to measure glycogen and free glucose in tissue homogenates, using spectrophotometry to detect levels of glucose in tissue samples. We inferred the amount of glucose stored in glycogen using reaction catalyzed by amyloglucosidase. A control reaction was without amyloglucosidase, this measured free glucose or not part of glycogen.

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