1 answer

Please explain briefly When making cDNA from mRNA, what is the appropriate primer to use? Choose...

Question:

please explain brieflyWhen making cDNA from mRNA, what is the appropriate primer to use? Choose one: O 5-TTTTTTTTTTTTTTT-3 O 5-CCCCCCCCCCCCCCC-3

When making cDNA from mRNA, what is the appropriate primer to use? Choose one: O 5'-TTTTTTTTTTTTTTT-3' O 5'-CCCCCCCCCCCCCCC-3' O 5'-UUUUUUUUUUUUUUU-3' O 5-GGGGGGGGGGGGGGG-3' O 5'-AAAAAAAAAAAAAAA-3'

Answers

The correct option is 1st option i.e., 5'-TTTTTTTTTTTTTTTTTTTTT-3'

Explanation -

RNA is a single stranded polynucleotide chain. The formation of cDNA ( complementary DNA ) from the help of mRNA as a template strand is known as Reverse Transcription. The mRNA consists of poly(A) tail at the 3' end and this advantage is being utilised in Reverse Transcription. During the initial step of Reverse Transcription oligo (dT) primer is added

(5'-TTTTTTTTTTTTTTTTTTTTT-3'). This primer binds to the poly (A) tail and the further complementary base pairing starts.

.

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