# LINEWEAVER-BURK plot 1. You perform the hydrolysis reaction as required in the lab experiment, and the...

###### Question:

LINEWEAVER-BURK plot 1. You perform the hydrolysis reaction as required in the lab experiment, and the final reading on your blood glucose meter for one of your samples is 385 mg/dL. You need your data to be in units of mm (millimolar, which is millimoles per liter, abbreviated as mmol/L). Show, in a step-by-step conversion, how you would convert 385 mg/dL to the proper answer in mM. 385 mg dlº= ?mm immoles 2. Assuming that the reaction occurred over a 30 minute time period, what is the rate of the reaction in mM/min?
3. You use a bone alkaline phosphatase enzyme to cleave the phosphate group from phenyl phosphate at various concentrations of phenyl phosphate, and measure the rate of the reaction. a. Using the data below generate a Lineweaver-Burk plot of the data using Excel. b. Calculate Km and Vmax for the enzyme. Show all your work. D Phenyl Phosphate (mm) 4.00 2.00 1.00 0.5 V. (mM/min) 1.18 0.91 0.67 0.45

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