1 answer

LINEWEAVER-BURK plot 1. You perform the hydrolysis reaction as required in the lab experiment, and the...

Question:

LINEWEAVER-BURK plot 1. You perform the hydrolysis reaction as required in the lab experiment, and the final reading on your
3. You use a bone alkaline phosphatase enzyme to cleave the phosphate group from phenyl phosphate at various concentrations o
LINEWEAVER-BURK plot 1. You perform the hydrolysis reaction as required in the lab experiment, and the final reading on your blood glucose meter for one of your samples is 385 mg/dL. You need your data to be in units of mm (millimolar, which is millimoles per liter, abbreviated as mmol/L). Show, in a step-by-step conversion, how you would convert 385 mg/dL to the proper answer in mM. 385 mg dlº= ?mm immoles 2. Assuming that the reaction occurred over a 30 minute time period, what is the rate of the reaction in mM/min?
3. You use a bone alkaline phosphatase enzyme to cleave the phosphate group from phenyl phosphate at various concentrations of phenyl phosphate, and measure the rate of the reaction. a. Using the data below generate a Lineweaver-Burk plot of the data using Excel. b. Calculate Km and Vmax for the enzyme. Show all your work. D Phenyl Phosphate (mm) 4.00 2.00 1.00 0.5 V. (mM/min) 1.18 0.91 0.67 0.45

Answers

Reading in blood glucose meter is 385 mg/dL

= 385 × ( 10-3 g/10-1 L)

= 3.85 g/L

Now molecular weight of glucose=180

So no of moles of glucose per litre = (3.85/180)

=0.0214

So concentration of glucose = 0.0214 M

= 0.0214 × 103 mM

= 21.4 mM

2. As the reaction has occured over 30 minutes so rate of reaction

= Change in concentration/ time

= (21.4/30)mM/min

=0.7133 mM/min.

.

Similar Solved Questions

1 answer
Discuss the current standards used to protect humans and animals in research. What specific things have...
Discuss the current standards used to protect humans and animals in research. What specific things have been put into place to provide this protection? How can we continue to conduct ethical research moving forward with the more that we know? PLEASE GIVE IN DEBPTH RESPONES...
1 answer
Gummy Bears and Skittles are slightly differentiated products. The demand for a bag of Gummy Bears...
Gummy Bears and Skittles are slightly differentiated products. The demand for a bag of Gummy Bears is qB = 80 – 5pB + 3pS, where pB is the price of a bag of Gummy Bears and pS is the price of a bag of Skittles. The demand for a bag of Skittles is qS = 50 – 4pS + 2pB. Under profitmaximizi...
1 answer
CommercialServices.com Corporation provides business-to-business services on the Internet. Data concerning the most...
CommercialServices.com Corporation provides business-to-business services on the Internet. Data concerning the most recent year appear below: Sales Net operating income Average operating assets $ $ $ 2,400,000 144,000 800,000 The following questions are to be considered independently Exercise 10-13 ...
1 answer
Blossom Industries presents you with the following information. Complete the table for the year ended December...
Blossom Industries presents you with the following information. Complete the table for the year ended December 31, 2022. The company depreciates all assets using the half-year convention. (Round answers to o decimal places, e.g. 45,892.) Date Purchased 2/12/20 Life in Years Salvage Value $20,200 Dep...
1 answer
How do you graph #y =2 abs(x-4)+ 1#?
How do you graph #y =2 abs(x-4)+ 1#?...
1 answer
Power points presentations on Health, Safety, and Nutrition. Must 25 slides presentations.
Power points presentations on Health, Safety, and Nutrition. Must 25 slides presentations....
1 answer
H2O + 1-butene
H2O + 1-butene...
1 answer
How do you divide #(x^3-2x^2-3x+2) / (x-1)#?
How do you divide #(x^3-2x^2-3x+2) / (x-1)#?...
1 answer
No need to explain, please I beg just solve everything, would be greatly appreciated (thumbs up)!...
No need to explain, please I beg just solve everything, would be greatly appreciated (thumbs up)! :) Required information Problem 12-4A Partnership income allocation, statement of partners' equity, and closing entries LO P2 [The following information applies to the questions displayed below...
1 answer
Acetaminophen has the formula C8H9NO2. a dose contains 0.500g A) how many moles does 0.500g contain?...
acetaminophen has the formula C8H9NO2. a dose contains 0.500g A) how many moles does 0.500g contain? B) how many molecules does 0.500g contain? C) how many C atoms are in 0.500g?...
1 answer
On the basis of extensive tests, the yield point of a particular type of mild steel-reinforcing...
On the basis of extensive tests, the yield point of a particular type of mild steel-reinforcing bar is known to be normally distributed with o = 100. The composition of the bar has been slightly modified, but the modification is not believed to have affected either the normality or the value of o. (...
1 answer
How do you graph #f(x) - 1/2x - 3# by plotting points?
How do you graph #f(x) - 1/2x - 3# by plotting points?...
1 answer
John wants to have $ 2,000,000 for retirement in 45 years. he invest in a mutual...
john wants to have $ 2,000,000 for retirement in 45 years. he invest in a mutual fund. paying an average of 955 each year compounded quarterly. How much should he deposit into his mutual fund?...
1 answer
9) (10 pts) A company's balance sheet showed the following amounts as of December 31: Cash $150 75 100 175 250 $7...
9) (10 pts) A company's balance sheet showed the following amounts as of December 31: Cash $150 75 100 175 250 $75 225 300 Payables Аccruals Notes Payable Long-term Debt Common Stock Retained Earnings Receivables Inventory Net Fixed Assets 450 300 Total Assets $1050 $1050 Total Liab & ...
1 answer
Find the present value if a cash flow of $400 is received at the end of...
Find the present value if a cash flow of $400 is received at the end of each quarter over the next 5 years with a discount rate of 8% p.a....
1 answer
1. According to Steven Johnson (2005), the complexity of video games, television programs, and the internet...
1. According to Steven Johnson (2005), the complexity of video games, television programs, and the internet have: a) played a role in decreasing intelligence b) played a role in increasing intelligence c) had no effect on intelligence, but have increased stress levels dramatically d) had no signific...
1 answer
Derek borrows $294,333.00 to buy a house. He has a 30-year mortgage with a rate of...
Derek borrows $294,333.00 to buy a house. He has a 30-year mortgage with a rate of 5.79%. After making 124.00 payments, how much does he owe on the mortgage?...