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Answer:
- Acetyl –CoA formed in the pyruvate dehydrogenase reaction condenses with the four-carbon compound oxaloacetae to form citrate with the elimination of coenzyme A. Since the product has three carboxyl groups, this pathway is referred to as the tricarboxylic acid cycle.
- This product then undergoes isomerization to form isocitrate. The enzyme called aconitase because the compound cis-aconitase is an intermediate of the reaction.
- Eliminates CO2 to form the 5-carbon dicarboxylic acid alpha-ketoglutarate. Oxidation also occurs, with electrons transferred from the substrate to NAD+.
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