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I need help doing **problem** **number 5** listed below. If someone could show the work required for this problem it would be a great help!

Below is a list of solutions you will need for the mini-prep. Make 5mL of each solution as a team and store each in a 15mL Falcon tube. Store solution 1 and 70% ethanol at 4 degrees Celsius. Solution 3 and Elution Buffer can be stored at room temperature. Show your calculations in the space provided below.

**1. Solution 1** (lysis buffer) is (25mM Tris-HCl, pH 8.0, 50mM glucose, 10mM EDTA)

✓ Recall that you have Tris and EDTA stock solutions

✓ You will be provided with a 3M glucose stock solution

**2. Solution 2** (Denaturing solution) is (0.2N NaOH, 1.0% SDS)

✓ **Do NOT make today** (it needs to be made fresh on the day of the mini-prep)

✓ You will be provided stocks of 1N NaOH and 10% SDS

**3. Solution 3** (Renaturing Solution) is 5M potassium acetate.

✓ Add 3mL of 5M potassium acetate to 0.575mL of glacial acetic acid and 1.425mL of water

**4. 70% ethanol** (v/v)

✓ You will be provided with 100% (200 proof) ethanol

**5. Elution Buffer (EB)** is (10mM Tris-HCl, pH 8.0)

✓ Recall that you have Tris stock solution

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Runner A crosses the starting line of a marathon and runs at an average pace of 5.6 miles per hour

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