# Explain briefly (and within the context of the plasmid you just created) the role of each...

###### Question:

1. Explain briefly (and within the context of the plasmid you just created) the role of each of the 10 elements that are in bold

it needs a “ColE1 origin” (E. coli origin of replication)

· it needs a “2μ ori” (yeast origin of replication)

· a AmpR gene (coding for the bacterial resistance against the antibiotic ampicillin) and its promoter.

· a yeast gene coding for the synthesis of uracil and its promoter.

· a yeast gene coding for the resistance against the antibiotic Nourseothricin, and that uses a GAL1 promoter and a TEF terminator.

· two restriction enzyme cut sites that will allow the subcloning of a sequence of interest somewhere in the plasmid.

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