# Did all of the additives denature the phycocyanin? Identify the additives that had no effect on...

###### Question:

Did all of the additives denature the phycocyanin? Identify the additives that had no effect on the protein and explain why they are different from the others. Did the additives that denatured phycocyanin exhibit differences in their potency? Use your data to justify your answer.
Bradford Test Observations Hneces Additive Observations Loss of blue color Bright blue Heat No change Loss of blue color after 20 drops Bright blue Isopropanol Loss of most blue color after 20 drops Bright blue Abetone Loss of blue color after 3 drops Bright blue Bleach No change N/A 0:1 M NEC Loss of most color and cloudy after 20 drops I MAC, Loss of blue color after 5 drops Bright blue Det Loss of blue color after 10 drops Bright blue 10 M HỌ. Loss of blue color after 8 drops Bright blue 10 MNOH
Sample Observations Greyish brown / no change Glycine Greyish brown / no change Tyrosine Bright blue Gelatin Bright blue Albumin Bright blue Phycocyanin Greyish brown / no change Sucrose

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