1 answer

Did all of the additives denature the phycocyanin? Identify the additives that had no effect on...

Question:

Did all of the additives denature the phycocyanin? Identify the additives that had no effect on the protein and explain why t

Bradford Test Observations Hneces Additive Observations Loss of blue color Bright blue Heat No change Loss of blue color afte

Sample Observations Greyish brown / no change Glycine Greyish brown / no change Tyrosine Bright blue Gelatin Bright blue Albu

Did all of the additives denature the phycocyanin? Identify the additives that had no effect on the protein and explain why they are different from the others. Did the additives that denatured phycocyanin exhibit differences in their potency? Use your data to justify your answer.
Bradford Test Observations Hneces Additive Observations Loss of blue color Bright blue Heat No change Loss of blue color after 20 drops Bright blue Isopropanol Loss of most blue color after 20 drops Bright blue Abetone Loss of blue color after 3 drops Bright blue Bleach No change N/A 0:1 M NEC Loss of most color and cloudy after 20 drops I MAC, Loss of blue color after 5 drops Bright blue Det Loss of blue color after 10 drops Bright blue 10 M HỌ. Loss of blue color after 8 drops Bright blue 10 MNOH
Sample Observations Greyish brown / no change Glycine Greyish brown / no change Tyrosine Bright blue Gelatin Bright blue Albumin Bright blue Phycocyanin Greyish brown / no change Sucrose

Answers

From the above data, one can see the effect of additives on anthocyanin denaturation. in the table, the additives distilled water and 0.1M Nacl have no effect on additives. These additives are different from other additives as distilled water has no chemical which can have any effect on anthocyanin. similarly 0.1M Nacl is the salt solution at low concentration.

These additives exhibit differences in their potency in denaturation of anthocyanin. from the data, you can see heat completely denatures the anthocyanin as immediate loss of blue colour occurs.

similarly only 3 drops of bleach denatures it.

only 5 drops of detergent denatures it.

8 drops of 1.0 M NaOH denatures it.

10 drops of 1.0 M HCl denatures it.

20 drops of isopropanol completely denatures it.

20 drops of methanol reduces the colour which means more methanol is required to denature it.

20 drops of 0.1M zncl2 also denatures but not fully.

from this data you can get the idea of potency of these additives

.

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